不同粒径的纳米ZrO 2 对废水污泥脱氮除磷性能和微生物群落的影响
发布时间：2024-04-20 15:23
最近,大量的合成纳米材料被广泛用于制药,电子,化妆品,诊断成像,光热疗法,核酸传递,催化和材料科学,环境修复以及清洁能源生产医疗设备,纺织等行业中。大量的合成纳米材料释放到市政污水处理厂中造成了污染。广泛用于市政废水处理的序批式反应器(SBR)传统上并不是以去除纳米颗粒而设计的。目前,ZrO2-NPs对SBR系统性能的影响仍然未知。在这项研究中,使用实验室规模的SBR系统研究ZrO2-NPs在不同粒径条件下(例如:200nm,100nm,50nm和15nm)对活性污泥性能及其特性和细菌群落动态的影响。实验室规模的浸没式SBR系统连续在纳米材料的胁迫下连续运行72天。在运行72天后发现,浓度为0.1g/L的ZrO2-NP(200nm,100nm,50nm和15nm)会对氮,磷和COD(化学需氧量)的去除产生不利影响。SBR-C对照组实验表示不添加NP的反应器中NH4+-N为(88.9±1.7%),但SBR-T中,NH4+-N的去除率显着...
【文章页数】：127 页
【学位级别】：硕士
【文章目录】：
中文摘要
ABSTRACT
List of Abbreviations
CHAPTER 1-INTRODUCTION
1.1 Significance of phosphorus (P) and Nitrogen (N) removal from wastewater
1.1.1 Treatment method of phosphorus-containing sewage
1.1.2 Biological nitrogen removal mechanism
1.2 Present uses of nano-enabled water treatment composites (NWTCs)
1.2.1 Physiochemical properties of ZrO2 nanoparticle
1.2.2 Size -dependent toxicity of nanoparticles
1.2.3 Negative impacts of nanoparticles on sewage water treatment
1.2.4 Fate of Nanoparticle release into the environment
1.3 Pertinence of nanoparticles in wastewater sludge
1.4 Modified NPs size induced effect on wastewater sludge
1.5 Mechanism of NPs toxicity on activated sludge system
1.5.1 Nano-bio interaction in wastewater and sludge environment
1.5.2 Impact of NPs on microbial diversity
1.5.3 Effects of NPs on biological phosphorus removal
1.5.4 Effects of NPs on nitrification and denitrification
1.5.5 Effects of NPs on organic-degradation microbial communities
1.6 Importance of present study
1.6.1 Motivation of research
1.6.2 Study gapes
1.6.3 Study objectives
CHAPTER 2-MATERIAL AND METHODS
2.1 The seed sludge sample collection (Tianjin WWTP facility)
2.2 Experimental Instruments
2.3 Experimental setup
2.4 Synthetic municipal wastewater feed
2.5 Operating conditions in the SBR system
2.6 Adjacent sludge influent flow distribution design
2.7 Analytical methods
2.7.1 Wastewater characteristics
2.7.2 Total Phosphorus
2.7.3 Chemical oxygen demand (COD)
2.7.4 NH4
+-N,NO3
-
2.7.5 Determination of polysaccharides (PS)
2.8 Sludge characteristics
2.9 Bacterial community dynamics
2.9.1 Steps of Gel Electrophoresis
2.9.2 Steps for clone sequencing
2.10 FISH (rRNA-targeted oligonucleotides)
2.10.1 Optimization of reaction conditions and process
2.10.2 Optimization of FISH hybridization conditions
2.10.3 DAPI counter staining
2.11 Scanning electron microscopy (Activated sludge morphology)
CHAPTER 3-SYNTHESIS OF ZrO2 NANOPARTICLES
3.1 Chemicals
3.2 Preparation method and material
3.3 Powder X-ray diffraction of pure ZrO2
3.4 FT-IR spectrum of pure ZrO2 (precursors substances)
3.5 XRD and SEM growth patterns of ZrO2 nanoparticle crystal formation
CHAPTER 4-RESULTS AND DISCUSSION
4.1 Reactor startup by using adjacent influent load distribution scheme
4.1.1 Sludge biomass density
4.1.2 Characteristics of Sludge during reactor startup
4.1.3 Pattern of DO (dissolved oxygen) and pH
4.2 Effect of ZrO2-NPs on wastewater treatment
4.2.1 Effect of ZrO2-NPs on Wastewater
4.2.2 Effect of ZrO2-NPs on MLSS and MLVSS
4.2.3 Effect of ZrO2-NPs on the formation of polysaccharide (PS)
4.2.4 Nitrification inhibition assay
4.4 Morphological changes on the sludge surface
4.5 Effect of ZrO2 NPs on Nitrifying and polyphosphate accumulating organisms
4.5.1 16SrDNA sequence analysis
4.5.2 Bacterial diversity when exposed at different ZrO2 NPs
4.5.3 Physiochemical parameteter changes with ZrO2 NPs exposure
4.5.4 Influence of ZrO2 NPs on phylum level abundance and functional diversity prediction in wastewater
4.6 FISH-Analysis (before and after nano-ZrO2 exposure)
CHAPTER 5-CONCLUSIONS
REFERENCES
ACADEMIC PAPERS PUBLISHED DURING THE STUDY FOR A MASTER SDEGREE
ACKNOWLEDGEMENT
本文编号：3959607
【文章页数】：127 页
【学位级别】：硕士
【文章目录】：
中文摘要
ABSTRACT
List of Abbreviations
CHAPTER 1-INTRODUCTION
1.1 Significance of phosphorus (P) and Nitrogen (N) removal from wastewater
1.1.1 Treatment method of phosphorus-containing sewage
1.1.2 Biological nitrogen removal mechanism
1.2 Present uses of nano-enabled water treatment composites (NWTCs)
1.2.1 Physiochemical properties of ZrO2 nanoparticle
1.2.2 Size -dependent toxicity of nanoparticles
1.2.3 Negative impacts of nanoparticles on sewage water treatment
1.2.4 Fate of Nanoparticle release into the environment
1.3 Pertinence of nanoparticles in wastewater sludge
1.4 Modified NPs size induced effect on wastewater sludge
1.5 Mechanism of NPs toxicity on activated sludge system
1.5.1 Nano-bio interaction in wastewater and sludge environment
1.5.2 Impact of NPs on microbial diversity
1.5.3 Effects of NPs on biological phosphorus removal
1.5.4 Effects of NPs on nitrification and denitrification
1.5.5 Effects of NPs on organic-degradation microbial communities
1.6 Importance of present study
1.6.1 Motivation of research
1.6.2 Study gapes
1.6.3 Study objectives
CHAPTER 2-MATERIAL AND METHODS
2.1 The seed sludge sample collection (Tianjin WWTP facility)
2.2 Experimental Instruments
2.3 Experimental setup
2.4 Synthetic municipal wastewater feed
2.5 Operating conditions in the SBR system
2.6 Adjacent sludge influent flow distribution design
2.7 Analytical methods
2.7.1 Wastewater characteristics
2.7.2 Total Phosphorus
2.7.3 Chemical oxygen demand (COD)
2.7.4 NH4
+-N,NO3
-
2.8 Sludge characteristics
2.9 Bacterial community dynamics
2.9.1 Steps of Gel Electrophoresis
2.9.2 Steps for clone sequencing
2.10 FISH (rRNA-targeted oligonucleotides)
2.10.1 Optimization of reaction conditions and process
2.10.2 Optimization of FISH hybridization conditions
2.10.3 DAPI counter staining
2.11 Scanning electron microscopy (Activated sludge morphology)
CHAPTER 3-SYNTHESIS OF ZrO2 NANOPARTICLES
3.1 Chemicals
3.2 Preparation method and material
3.3 Powder X-ray diffraction of pure ZrO2
3.5 XRD and SEM growth patterns of ZrO2 nanoparticle crystal formation
CHAPTER 4-RESULTS AND DISCUSSION
4.1 Reactor startup by using adjacent influent load distribution scheme
4.1.1 Sludge biomass density
4.1.2 Characteristics of Sludge during reactor startup
4.1.3 Pattern of DO (dissolved oxygen) and pH
4.2 Effect of ZrO2-NPs on wastewater treatment
4.2.1 Effect of ZrO2-NPs on Wastewater
4.2.2 Effect of ZrO2-NPs on MLSS and MLVSS
4.2.3 Effect of ZrO2-NPs on the formation of polysaccharide (PS)
4.2.4 Nitrification inhibition assay
4.4 Morphological changes on the sludge surface
4.5 Effect of ZrO2 NPs on Nitrifying and polyphosphate accumulating organisms
4.5.1 16SrDNA sequence analysis
4.5.2 Bacterial diversity when exposed at different ZrO2 NPs
4.5.3 Physiochemical parameteter changes with ZrO2 NPs exposure
4.5.4 Influence of ZrO2 NPs on phylum level abundance and functional diversity prediction in wastewater
4.6 FISH-Analysis (before and after nano-ZrO2 exposure)
CHAPTER 5-CONCLUSIONS
REFERENCES
ACADEMIC PAPERS PUBLISHED DURING THE STUDY FOR A MASTER SDEGREE
ACKNOWLEDGEMENT
本文编号：3959607
本文链接：https://www.wllwen.com/wenshubaike/qiuzhijiqiao/3959607.html