Antibiotics and Cu(Ⅱ):Co-sorption and Acetylcholin Esterase

发布时间：2024-04-18 04:13

　　污染物的共存是环境中一个普遍的现象。现在土壤和水体环境中都能检测到抗生素和重金属的存在,它们之间的相互作用可能会影响彼此的环境行为和风险。在有关抗生素和重金属的环境行为研究中,其吸附行为和生物毒性是研究的主要内容。土壤是污染物环境行为的重要介质,而有机质作为土壤的重要组份,直接暴露在土壤颗粒的外表面,关系到污染物的吸附行为。在本研究中,不同有机质含量的土壤被选为吸附剂,研究抗生素(磺胺甲恶唑,SMX和氧氟沙星,OFL)与重金属(Cu2+)在土壤中的共吸附行为。研究结果表明,抗生素和Cu2+的相互作用既可以促进、也可以抑制彼此在土壤中的吸附。在比较主要吸附质和共吸附质的吸附系数时表明,抗生素和Cu2+相互促进的情况发生在主要吸附质吸附系数高的时候(主要吸附质浓度低)。而抗生素和Cu2+相互抑制的情况发生在主要吸附质吸附系数下降到一定数值时(数值与土壤颗粒的性质有关)。这可能是由于吸附质浓度低时,抗生素和Cu2+占据的是各自的高能吸附位点(对于抗生素来说,其高能位点可能是土壤颗粒上的憎水区域；对于Cu。+来说,其高能位点可能是内层络合位点),Cu2+与被吸附的抗生素络合、抗生素通过被吸附的...

【文章页数】：127 页

【学位级别】：博士

【文章目录】：
Abstract
摘要
Chapter 1 Introduction
    1.1 Background and significance
    1.2 Objectives of the study
    1.3 Contents of the study
    1.4 The organization of this thesis
Chapter 2 Literature Review
    2.1 The interaction between antibiotics and HMs
    2.2 Co-sorption behavior of antibiotics and HMs
    2.3 The toxicity of antibiotics and HMs
    2.4 Key questions need to be discussed in this study
Chapter 3 Research Method
    3.1 Introduction
    3.2 Sorption experiments
        3.2.1 Sorbates
        3.2.2 Sorbents
        3.2.3 Collection and pretreatment of soils
        3.2.4 Batch sorption experiments
        3.2.5 Chemical quantification
        3.2.6 Data processing method
    3.3 Enzyme inhibition experiments
        3.3.1 Preparation of AChE
        3.3.2 Chromogenic method for enzyme activity quantification
        3.3.3 Inhibition experiments
        3.3.4 Data processing method
        3.3.5 Enzyme structural analysis
Chapter 4 Co-Sorption of Ofloxacin and Cu in Soils before and after Organic Matter Removal
    4.1 Introduction
    4.2 Cu sorption on different particles
    4.3 OFL sorption on different particles
    4.4 Co-sorption of OFL and Cu
    4.5 Conclusion
Chapter 5 Co-sorption of Sulfamethoxazole and Cu on Hydroxylized and Graphitized Carbon Nanotubes
    5.1 Introduction
    5.2 SMX-Cu complexation as an affected by pH
    5.3 Sorption of SMX or Cu individually on MWCNTs
    5.4 Co-sorption of SMX and Cu on MWCNTs
    5.5 Conclusion
Chapter 6 Inhibition of Acetylcholin Esterase by coexisting Cu Ofloxacin
    6.1 Introduction
    6.2 Inhibition of AChE activity by OFL or Cu
    6.3 Secondary structural change of AChE as an affected by OFL or Cu
    6.4 Inhibition of AChE activity by coexsiting Cu and OFL
    6.5 Conclusion
Chapter 7 Conclusions and Prospectives
    7.1 Conclusions
    7.2 Perspectives
Acknowledgements
Reference
Appendix 1 List of Publications and Prizes
Appendix 2 List of Symbols and Abbreviations



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