中国大鲵ERα、P450arom基因的克隆及不同发育期4种性别决定相关基因的表达分析

发布时间：2024-04-24 03:22

　　中国大鲵(Andrias davidianus)是我国特有的有尾两栖动物,也是全球现存两栖类中体型最大的古老物种,属于国家Ⅱ级重点保护野生动物,具有重要的经济、科研和生态价值。然而,近年来由于栖息地的破坏、过度捕杀以及传染病等因素,导致中国大鲵种群数量急剧下降。为了更好地保护这一古老物种,我国科研人员开始了中国大鲵的人工驯养繁殖研究,先后形成了中国大鲵全人工繁育(Captive Breeding)、原生态繁育(Natural Eco-breeding)、仿生态繁育(Imitated Natural Eco-breeding)等3种人工繁殖技术。随着人工繁育技术的突破,中国大鲵人工种群数量剧增。然而,在人工较高水温饲养条件下,幼鲵绝大部分发育成为雄性,造成中国大鲵人工种群性别比例严重失调。为了解决此问题,在幼鲵性腺分化的敏感期,使用一定浓度的外源雌二醇断续暴露40 d,又使得幼鲵已连续4年全部分化为雌性。以上现象表明,中国大鲵性腺分化受到环境水温与外源雌二醇的直接影响。本文在中国大鲵首次克隆了雌激素受体(estrogen receptor alpha, ERa)、细胞色素P450芳香化酶...

【文章页数】：78 页

【学位级别】：硕士

【文章目录】：
摘要
Abstract
Chapter 1 Review
    1 Introduction of the Chinese giant salamander
    2 Sexual size dimorphism
    3 Introduction of the four genes
        3.1 ERα
        3.2 P450arom
        3.3 Sox9
        3.4 Dmrt2
Chapter 2 Molecular cloning of ERα and P450arom in the Chinese giantsalamander
    1. Material and method
        1.1 Materials
        1.2 Methods
    2. Result
        2.1 The result of 3'race
        2.2 Molecular cloning and sequence analysis of the Chinese giant salamander ERα
        2.3 Molecular cloning and sequence analysis of Chinese giant salamander P450arom
        2.4 Tissue distribution pattern of ERα and P450arom
    3. Discussion
Chapter 3 Expressions of ERα, P450arom, Sox9 and Dmrt2 during thedevelopment of the Chinese giant salamander
    1. Material and method
        1.1 Materials
        1.2 method
    2. Result
        2.1 Head-body length,tail length and body weight of the Chinese giant salamander during development
        2.2 Histology of the Chinese giant salamander gonads during the development
        2.3 Expression of ERα,P450arom,Sox9 and Dmrt2 in gonad,brain and liver of the Chinese giant salamander during development
    3. Discussion
        3.1 Sexual dimorphism in the Chinese giant salamander
        3.2 Possible role of P450arom during development
        3.3 Possible role of ERα during development
        3.4 Possible role of Sox9 during development
        3.5 Possible role of Dmrt2 during development
Summarise
References
致谢
攻读硕士期间的研究成果



本文编号：3963155