IgA Fc受体Fc α RI在过敏性紫癜患儿中的表达及意义

发布时间：2019-06-11 22:00

【摘要】：目的:观察急性期过敏性紫癜(Henoch-Schonlein purpura,HSP)患儿外周血及皮损组织IgA Fc受体FcαRI的表达,并探讨FcαRI与炎症相关细胞因子白细胞介素-6(interleukin-6,IL-6)、肿瘤坏死因子-α(tumor necrosis factor,TNF-α)的关系。方法:纳入急性期HSP患儿50例,其中单纯型HSP 30例,紫癜性肾炎型(Henoch-Schonlein purpura nephritis,HSPN)20例,健康对照20例。采用Sandwich固相ELISA法检测血清可溶性IgA Fc受体(s FcαRI-IgA)水平;免疫组化及Western blot分别检测20例HSP患儿皮损组织中FcαRI的表达和分布,直接免疫荧光检测皮损组织中IgA、C3、纤维蛋白原(fibrinogen,Fib)的沉积;ELISA检测患儿血清细胞因子IL-6、TNF-α的水平。结果:(1)急性期HSP和HSPN患儿血清IgA[(2.32±1.00)g/L,(2.42±1.02)g/L]、C3[(1.17±0.33)g/L,(1.11±0.19)g/L]较正常对照明显升高(F=14.000,P=0.000;F=8.227,P=0.000),HSPN组血清s FcαRIIgA水平[(5.75±5.07)pg/mL]较正常对照组[(3.09±2.08)pg/mL]升高(F=2.891,P=0.022)。(2)FcαRI在急性期HSP患儿皮损表皮及真皮中性粒细胞、血管内皮细胞、附属器周围沉积,组化染色强度(HSCORE=205.00±41.86)及FcαRI蛋白表达(FcαRI/beta actin=0.23±0.07)较正常对照组明显增高(t=8.276,P=0.000;t=3.274,P=0.004)。(3)急性期HSP和HSPN患儿PBMC上清中TNF-α水平[(4.75±2.59)pg/mL,(6.37±2.87)pg/mL]较正常对照组[(3.12±2.01)pg/mL]增高(F=8.309,P=0.028,P=0.000),HSPN组升高较HSP更显著(P=0.030)。结论:IgA Fc受体参与急性期HSP发病,FcαRI可能作为急性期HSP的重要分子标记,但FcαRI通过何种机制参与HSP发病仍需深入研究。
[Abstract]:Objective: To observe the expression of Fc-RRI in peripheral blood and skin lesions of children with Henoch-Schonlein purpuura (HSP), and to explore the relationship of Fc-RI with inflammation-related cytokine interleukin-6 (IL-6), tumor necrosis factor (TNF-1). Methods:50 cases of HSP in the acute stage were included. Among them,30 cases of simple HSP,20 cases of Henoch-Schonlein purpuura nephatis and HSPN, and 20 healthy controls. Serum soluble IgA Fc receptor (s-Fc-RI-IgA) was detected by means of Sandwich solid-phase ELISA. The expression and distribution of Fc-RRI in the lesions of 20 patients with HSP were detected by immunohistochemistry and Western blot, and the deposition of IgA, C3, and Fib in the skin was detected by direct immunofluorescence. The levels of serum cytokines IL-6 and TNF-1 in children were detected by ELISA. Results: (1) The serum IgA[(2.32-1.00) g/ L, (2.42-1.02) g/ L], C3[(1.17-0.33) g/ L], (1.11-0.19) g/ L] in the acute phase HSP and HSPN were significantly higher than that in the normal control (F = 14.000, P = 0.000; F = 8.227, P = 0.000). The level of serum s-Fc[(5.75-5.07) pg/ mL] in the HSPN group was higher than that in the normal control group[(3.09-2.08) pg/ mL] (F = 2.891, P = 0.022). (2) The expression of Fc-RI (Fc-RI/ beta-actin = 0.23-0.07) was higher than that in the normal control group (t = 8.276, P = 0.000; t = 3.274, P = 0.004). (3) The level of TNF-[(4.75-2.59) pg/ mL, (6.37-2.87) pg/ mL] in the PBMC of patients with acute phase HSP and HSPN was higher than that in the normal control group[(3.12[2.01) pg/ mL] (F = 8.309, P = 0.028, P = 0.000), and higher in the HSPN group (P = 0.030). Conclusion: IgA Fc receptor is involved in the pathogenesis of HSP in the acute stage, and the Fc-RRI may be an important molecular marker for HSP in the acute stage, but the mechanism of the Fc-RI in the pathogenesis of HSP is still in-depth study.
【作者单位】：重庆医科大学附属儿童医院皮肤科;重庆医科大学附属儿童医院肾内科儿童发育疾病研究教育部重点实验室儿科学重庆市重点实验室儿童发育重大疾病国家国际科技合作基地;
【基金】：国家自然科学基金资助项目(编号:81301362)
【分类号】：R725.5

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